FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14782710
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA246670.166863856491807No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA182120.12319797926090682No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT167020.11298334337885273No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA156850.10610368464239642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA25250.033.5035674
CCCGTAT33500.029.8986321
TATCGAA35750.024.5836015
CGCGTAT2052.9764124E-822.9383751
CGTATCG54900.022.7713743
CCCGTCG27900.022.0792141
CCGTATC60950.021.436362
CGCGTAA30550.018.7786851
CGTACGC49550.018.6854065
AGTTTAC208700.018.01414994
CCGTACG52350.017.865554
ATCGAAG74850.017.517316
GCGTAAC34050.017.3913542
TACGCCA55550.017.342737
TTCGCGC12750.016.9549037
GTACGCC54900.016.9491466
TCCGTAC56650.016.7583543
CGCTAGT3151.5062142E-716.4209651
CTCTTAT111250.016.1888221
CCCGATT10850.016.0357231
Produced by FastQC (version 0.11.5)