FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11524659
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGC14200.023.4145765
CCGTACG14350.023.1698234
CCCGTAT17450.021.310681
TGTCGAG14000.020.3563445
CTCGTTT45800.019.5699861
GTACGCC18400.019.102516
GTATCGA14700.018.4176454
GTCGAGG16550.018.080866
TTCGAAA32050.017.7840146
ACCCGTT19750.017.5562742
TACGCCA19900.017.4239397
TCCGTAC19850.017.2285423
CGAAAGG30450.017.1586048
ACGTACG7900.016.83478252-53
CGTATCG26550.016.6377663
CCCGTCT32700.016.329331
CGTACGA8300.016.309654-55
CCGTATC31250.016.2633612
GTGTCGA22350.015.9389714
TCGACCG10950.015.61582630-31
Produced by FastQC (version 0.11.5)