FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9428370
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA94360.10008092597129725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGC9800.041.682235
CCGTACG10150.040.244914
GTACGCC14300.028.8975986
TCCGTAC14450.028.5976263
TACGCCA15800.026.7554027
CCCGTAT14400.024.8203331
ACCCGTT18200.024.2711352
CACCCGT19900.023.2288381
TGTCGAG12300.023.169935
GTATCGA11650.022.0164054
GTCGAGG13900.020.1611736
TCCGCGG17300.018.120762
CGTATCG24000.018.0097923
TATCGAA20350.017.5055355
TTCCGTA24700.017.307092
TATCGCA8050.017.111218
CGTACGA7100.017.05926754-55
CTCGTTT36650.017.033591
CTCCGCG20950.016.8328061
CTCCGTT30650.016.3255441
Produced by FastQC (version 0.11.5)