FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10785852
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT107870.10001064357270989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAG14750.029.6260995
CGTACGC17250.026.4338745
GTCGAGG17800.025.3502526
CCGTACG17850.025.0131454
ACCCGTT23200.024.3633862
TCGAGGG22050.022.4028557
TCCGTAC21650.021.061633
ACGTACG9050.020.99375252-53
GTACGCC22000.020.9424616
CACCCGT27700.020.6443791
CCCGTAT17800.020.6144181
TACGCCA21900.020.6043157
CGTACGA10150.020.35644154-55
CCCGGTT11850.019.7050741
CCCGTCT28800.019.1940161
GTATCGA11950.019.0788424
GCTACGT13200.018.17172450-51
GTGTCGA27200.017.6372624
TACGTAC13800.017.20955352-53
CTCGTTT44550.016.9008961
Produced by FastQC (version 0.11.5)