FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10785852
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA165070.1530430790261168No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT137840.12779704375695122No Hit
CTACAAGGCTCGGAGCTCCGGGCACTCAGACATCATGAGTTGGTCCTTGC113730.10544368678524423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT7300.038.942121
TAGAGGC63050.027.9544563
TCACCCG34100.027.9276813
ACCCGGC35300.027.11345
GTTAGTT64550.025.9835361
CATTCGG20300.024.740884
ACGACGT9550.024.3117457
CCTAGAG75250.024.114791
ACTAGCG11300.023.900325
CTAGCGT11550.022.9729826
GCATTCG21250.022.7423063
ACCGTTG13350.022.7139113
TACCCTA74900.022.2697079
CCGTTGA12600.022.1863944
CTAGAGG83350.021.373492
CGGCCCG46850.021.3406858
CCGGGTA48800.021.10320742-43
CGGGTAC52000.020.67156842-43
CGACCTA11750.020.1621784
TAGCGTC13600.019.8590937
Produced by FastQC (version 0.11.5)