FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10943723
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT112710.10299054535645685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT14000.031.658911
CGTACGC17550.025.9819855
CGCGTAT1309.775204E-625.6623121
CCGTACG18150.025.1230774
TGTCGAG14900.024.8648915
GTATCGA12650.023.6552894
ACCCGTT26600.022.6777692
GTCGAGG18450.020.852926
GTACGCC22850.020.579146
CACCCGT31400.020.4901521
TCGAGGG19750.019.7208217
TACGCCA23450.019.6474917
TCCGTAC23800.019.5581323
CGTACGA9450.018.59722554-55
CCCGTCT30350.017.901411
ACGTACG9500.017.74937252-53
CTCGTTT43800.017.7359541
TCGCGTG1650.001465239217.2721162
CCGGGAT26950.016.799871
TCGACCG9650.016.48905430-31
Produced by FastQC (version 0.11.5)