FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10943723
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA172620.15773425551798048No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT141680.12946234110640412No Hit
CTACAAGGCTCGGAGCTCCGGGCACTCAGACATCATGAGTTGGTCCTTGC115660.10568615451981012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT7350.039.9744261
TAGAGGC60650.027.5038623
TCACCCG32350.027.2471143
GTCGTAA7600.026.1892765
ACCCGGC35250.025.0058525
CCTAGAG70600.024.7013681
ACCGTTG13250.024.3206353
GTTAGTT70900.023.7947771
CATTCGG20750.023.066684
ACGACGT10550.022.909287
GCATTCG21600.022.8171543
TACCCTA76150.022.1564249
TAGGTCG5200.021.8720633
CTAGAGG76650.021.4533542
CGACCTA12250.021.2769054
CCGTTGA14600.021.0980514
ACGCACG16600.020.75148852-53
GCTCGGA57250.020.3641038
CTCGATT27250.020.30526548-49
TCGTAAG9900.020.10496
Produced by FastQC (version 0.11.5)