FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12042026
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT2381881.9779728095587903No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1565691.300188190924019No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC644920.5355577209349988No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG510430.4238738564424292No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA417090.34636198260990303No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT399470.3317298932920424No Hit
CACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATG325940.2706687396290292No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG230010.191006064926284No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA185430.15398571635703162No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA168270.13973562256052263No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC151450.12576787327979527No Hit
CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGC146650.12178183305699555No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGA146030.12126696952821725No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG130760.10858637906943566No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA128280.10652692495432246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG373500.066.926413
CCTCACC395900.063.5347181
ACCCGGC397800.062.7314535
CACCCGG408050.061.22514
CCAGTAG270050.058.276575
CTCACCC432200.057.9134332
CGGCCCG436600.057.1806838
GCCAGTA278800.056.3963624
TAGCATA283550.055.5897069
CAGTAGC284450.055.4596526
GTAGCAT289750.054.561958
CCTGCCA292400.053.9027021
TGCCAGT304400.051.7154923
AGTAGCA306850.051.597077
CCGGCCC536950.047.0753867
CTGCCAG338100.046.5467532
CCCGGCC556850.045.0776256
GGCCCGG569450.043.9337049
CACTCGG77400.042.6734241
CGGACAC406100.039.0781112-13
Produced by FastQC (version 0.11.5)