FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12753519
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA533130.4180258013494158No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA325880.25552163289206686No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG300990.23600545073089238No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA244820.191962704568049No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG240080.1882460832966964No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT225470.17679042152993224No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG219940.17245436338002082No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT209060.16392338459683164No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA201580.15805833668338912No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG189060.14824143830420453No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA168870.1324105135217974No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC167470.1313127772813135No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA159850.12533795574382256No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC157820.12374623819512089No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC148930.11677561306804812No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA141290.11078510958426455No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA131200.10287356767963414No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT130820.10257561070007423No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC130150.10205026549927121No Hit
CGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAG129870.10183071825117444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAG17800.044.5630235
CCCGTCG70000.044.0507051
CGCGTAA150400.036.7901041
GCGTAAC155700.036.271992
GTATCGA24000.035.4258234
GTCGAGG22400.035.4116866
CGTAACT162450.034.9402773
CCCGTAT29450.033.337161
ACTAGTT174400.033.0908557
GTAACTA172100.033.0363044
GTTACGA12000.031.665544
TAACTAG187650.030.2986835
CTAGTTA188800.030.2902368
TATCGAA29400.029.7268335
TAGTTAG199100.029.4627889
ACGACTT33950.029.1991481
TTACGAC13200.029.146695
AACTAGT206750.028.0739736
CCGTCGG120350.025.9296972
CTCGCGT6850.025.7429181
Produced by FastQC (version 0.11.5)