FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12412890
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA183950.14819272546522205No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA153400.12358121275545018No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG144250.1162098431549784No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT140470.1131646216151114No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT136960.11033691589952058No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG133390.10746087333409061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA41550.037.495444
CCCGTAT44700.034.9905621
CGTACGC26900.033.7253725
TATCGAA48900.032.151025
CCGTACG28550.032.1090054
TGTCGAG14700.031.6653585
GTCGAGG15800.030.9639116
TACGCCA30250.029.9904947
TCCGTAC30300.029.9410063
GTACGCC30350.029.7351786
CGCGTAA12200.029.3147451
CGTATCG66550.027.1927153
CCGTATC68300.026.2873482
GCGTAAC14300.025.5758672
TCGAGGG19350.025.0377257
TTCCGTA39050.023.3537082
ACGTACG9800.023.26434552-53
ATCGAAG88600.021.6582476
CGTACGA11050.021.06247754-55
CGGTAAT8900.019.8242321
Produced by FastQC (version 0.11.5)