FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12331168
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC309950.2513549405863257No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC290290.23541160091241964No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG241870.19614524755481394No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA188690.1530187570228546No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT166590.13509669157049845No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA166250.13482096748661604No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT150280.121870045076022No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC138260.11212238775759116No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC137450.11146551567540074No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT135420.10981928070398522No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA133600.10834334590202647No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC132710.10762159756480488No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA131360.10652681076115417No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG128100.10388310336863467No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA125980.10216388261030909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT19200.065.908411
ACCGTTG23850.053.058983
GACCGTT25950.048.9476362
CCGTTGA25550.048.7866364
ACATGCC107400.031.817442
CGCAAAA56700.031.5129491
CCGTATG47400.029.5971154
GTAGGTC35550.028.7970772
ACCCGGC38700.028.2902665
CGTATGG50800.027.4296135
ACCGTAT51200.027.4004553
TAACCCC63950.026.8301729
GTTAGTT65450.026.4310231
TGCCTAT125700.025.9034445
CTACCGT55850.025.712851
CGGCCCG44600.024.6547018
AACCCAC126750.024.3801125
CCCAAAC144050.024.11691
CTCGATT28100.024.08351348-49
CATGCCT137550.023.981963
Produced by FastQC (version 0.11.5)