FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9941983
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT269630.271203441003671No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT174000.17501538676942013No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT148490.14935652173213332No Hit
CTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCTTTA119320.1200162985593518No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT118440.11913116326994323No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT117230.11791410224700645No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT102230.10282656890481506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT8000.036.728911
TCACCCG29500.025.5435623
CGACCTA7200.023.0384274
CCGTTGA12500.022.74884
ACCGTTG12800.022.2150653
GTCAACC62150.021.5047286
ACCCGGC36500.021.2945375
GACCGTT13150.021.2633932
CGGCCCG36200.021.0798428
CTCGATT23700.020.13788848-49
ACGCCTT140850.019.6836382
GTCGTAA8000.019.549755
CGTTGAC14850.019.4679685
CGCGGTC9550.017.8650991
ATTCCGT27050.017.82715852-53
CGCCTTT155500.017.798723
CACGCCT161050.017.7444671
CACCCGG43300.017.5125484
TCAACCC77050.017.4699367
GTTAGTT50250.017.353541
Produced by FastQC (version 0.11.5)