FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9929075
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA172540.17377248132378897No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG141200.14220861459904371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGC10150.022.4622945
CCGTACG10550.021.6106434
CCCGTAT13900.021.5975461
CTCGTAT12850.021.1373421
GTATCGA10700.019.5320474
TGTCGAG10750.018.9993555
TTCGCGC7650.018.626827
ACGTACG5350.018.20031752-53
CGTACGA5650.017.6542754-55
GTCGAGG12750.017.509216
CTTCGCG9650.017.2273946
CTCCGAT25000.017.1546231
ACCCGTT16100.017.1112212
GTGTCGA12750.016.3916024
TAAACCG109950.016.0271979
CCCGTCT28500.015.8839091
TACGCCA14700.015.8327977
GTACGCC14800.015.7258196
TCGACCG6500.015.345633530-31
CGACGAA79850.015.28752144-45
Produced by FastQC (version 0.11.5)