FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10084417
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA175160.1736937296424771No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG144110.14290365025563698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGC10400.030.5998865
CCGTACG10600.030.0225354
CCCGTAT14850.024.71111
GTACGCC14250.023.3325166
TACGCCA15650.020.6382547
CGTACGA4400.019.43113554-55
TCCGTAC17200.019.3307173
TGTCGAG9550.018.4024965
TAAACCG109050.017.6839289
GTATCGA12150.017.5919744
CCGTATC25350.017.0506862
CGTATCG20200.016.9301013
TCGACCG6250.016.71941430-31
CTTACTT76500.016.5709721
CCCGTCT28850.016.5189341
ACCCGTT16750.016.4471842
ACGTACG5150.016.14021152-53
CTCCGTT22250.016.06421
CTCGGAT20450.015.6138251
CGACGAA81150.015.07186844-45
Produced by FastQC (version 0.11.5)