FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10820212
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA222710.20582776012152074No Hit
CTTACTTTCAACAAAATCAGCAGCTAATGAAGGCAAGTCAGCAGGCATCT143600.1327145900653333No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG138420.12792725318136094No Hit
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA131260.12131000760428724No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA125530.11601436275000897No Hit
CTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCATCCA118000.10905516453836579No Hit
CTCATGGTAGGCTGAAATGCTTTTAAATGTGATGTTATAAGCCTAAGGCA117650.10873169582998929No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA108410.10019212192884945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT26300.036.7947271
GTATCGA21400.036.400494
TATCGAA26350.030.463745
CGTATCG34600.027.3183293
CGTACGC14300.027.2367295
TTCGCGC8750.027.1418297
CCGTACG14850.025.9081084
CCGTATC39650.025.635852
ATCGAAG46750.022.2504926
TGTCGAG10900.022.2239285
TCCGTAC17800.021.614353
GTACGCC17950.021.4337276
ACCCGTT27350.020.8401582
TACGCCA18900.020.6076837
CCCGTCT33000.019.9347341
ACGTACG6150.019.3082152-53
CGTACGA6400.019.29614354-55
TCGAAGG54850.019.2244327
CTTACTT88750.019.1754321
GTCGAGG14300.018.6006936
Produced by FastQC (version 0.11.5)