FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004829256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11957024
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1899271.5884136387114385No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT548400.45864255185905795No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG539790.45144176343545017No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG470950.39386890918676754No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC449420.37586275648522577No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC405250.33892212644216485No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC390240.3263688355898592No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA298570.24970260158380547No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT296430.24791285858420958No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC293980.24586385374822362No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG290790.2431959658189195No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT274130.22926273293421506No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG254830.21312159279767273No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT253220.2117751039054534No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT249520.20868068843886237No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC245260.20511792900976028No Hit
CGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTG234350.19599358502583922No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC224590.18783101882207479No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG220120.18409263040703105No Hit
CCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCA211470.17685838884324395No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT201250.16831111152741687No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA197020.16477344195344928No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA192950.16136958494019918No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG189000.15806608734748714No Hit
CCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTC187710.1569872235767027No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTC179580.15018787283524732No Hit
CGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGG174380.1458389646119302No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA168730.14111370856159525No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT167720.14026901677206635No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC158610.1326500640962166No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT158520.132574794530813No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCA140620.11760451430054837No Hit
CACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCG140130.11719471333335117No Hit
CCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG140060.11713617033803729No Hit
CTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCAT138700.11599876357193897No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA136600.11424247371252245No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT135270.11313015680155865No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG133260.11144913650754569No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT130900.10947540123696332No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA130710.10931649882111134No Hit
CAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCT129620.10840490075122372No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT127020.10623044663956517No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA126070.10543593456030531No Hit
CACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT122730.10264259735532856No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA121010.10120411232761596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)