FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10594847
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA307850.2905657816483806No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC230270.21734150573387234No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA225650.2129808953352512No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG216510.20435406004447257No Hit
CTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCA193970.18307956688756336No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG181010.17084720524987287No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA172800.1630981551692063No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC168890.15940768186647716No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG164860.15560394595599164No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT161590.1525175398946299No Hit
CTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTTGC150230.141795346360358No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG137490.12977063283688758No Hit
CTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCATCCA135210.12761864328951614No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT132530.12508911171629No Hit
CTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCAT132250.12482483229819175No Hit
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC129310.1220498984081601No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT129040.12179505754070824No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA127680.12051141465280245No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG118920.11224324428658573No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACGAGCTCAACAAGTGCAGTTT112630.10630639593002145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGA15650.040.0624474
TTACGAC15900.040.035
CCCGTCG44100.038.9081461
GTATCGA38650.036.0077484
CCGTACG20900.035.6805274
CGTACGC20900.035.6805275
TGTTACG17750.035.590263
CCCGTAT41550.034.8721851
ACGACTT39450.034.5537871
TACGACT18800.033.8551566
TATCGAA42100.033.5082865
TCCGTAC23000.032.629253
GTACGCC23750.031.3988636
TGTCGAG10750.029.6035775
TACGCCA25400.029.5461737
CGTATCG58800.026.8995343
GTCGAGG12250.026.7541316
CCGTATC59500.026.1040952
CGCGTAA90650.025.0274011
GCGTAAC94550.024.7161852
Produced by FastQC (version 0.11.5)