FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004829268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10594847
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT933160.8807677921162995No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG605880.5718629065620296No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG317960.30010815635185667No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC312010.2944922187172689No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT280420.26467583722539834No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC279760.2640528928827382No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC224500.21189546201091908No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC221660.2092149136273511No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT200510.1892523790102868No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC195810.18481626020649472No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA195250.18428770137029823No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT189280.17865288663441767No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA187890.17734092809457278No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC180280.17015819105268815No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC180130.17001661279299268No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA168470.1590112627393298No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT168250.15880361462510972No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA159230.1502900419420875No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG154990.1462880964680283No Hit
CAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCCAAGGCAA143730.13566028844022004No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC127700.12053029175409517No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC123610.11666992453973145No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT123140.11622631265935224No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT122630.11574494657638756No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT120570.11380060514323614No Hit
CAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCCAAGGCAA119360.11265854051502584No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA114920.1084678240280393No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC114870.10842063127480744No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG112750.10641965853777785No Hit
CTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCAT111070.10483398202918834No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT110830.10460745681367555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)