FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004829270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10542986
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT931020.8830705077290248No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG608690.5773411820901593No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG315600.2993459348233982No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC307960.292099410925899No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC284580.26992353020292353No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT279850.26543713517214196No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC223940.21240661801125413No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC221450.21004485825932045No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT199120.188864900323305No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC198840.188599320913449No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA190470.18066039355453947No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT189700.1799300501774355No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA182840.17342335463596367No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC181770.17240846189115683No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC178130.1689559295630289No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA170160.16139640136105654No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT169370.1606470880261057No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA160320.15206318210040307No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG154410.1464575595566569No Hit
CAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCCAAGGCAA142960.13559725868933148No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC127240.12068687182170212No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT124630.11821129232268733No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC123330.11697824506264165No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT122180.11588747248644739No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT119960.11378180716544631No Hit
CAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCCAAGGCAA118690.11257721484217091No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA113680.1078252404015333No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC113590.10773987559122246No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG113470.1076260558441413No Hit
CTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCAT111810.10605154934285221No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT109000.10338627026536884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)