FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11635307
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT923080.7933439143462222No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG760800.653871874631241No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC385540.33135352595337625No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG306330.2632762504676499No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC298220.25630608629407026No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT268660.23090065436176285No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC209350.1799264944190987No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC198970.1710053718393507No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA194800.16742145265268893No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT193160.16601194966321045No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT182220.15660953337973807No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA178230.15318031574070198No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC166350.14297001359740658No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA164940.14175818480767202No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC160660.1380797257863501No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG160160.13764999926516766No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT157530.13538963776374788No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA153450.13188306935089894No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT151500.13020713591828734No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC145530.12507620125536867No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCA140340.12061563996549468No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC137340.11803728083839989No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG130100.11181484081167775No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA125740.10806762554696665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT29400.064.962931
CACCCGG160850.061.5501374
ACCCGGC161850.061.4051555
TCACCCG163000.061.0578423
ACCGTTG32500.059.058373
GACCGTT32900.058.052232
CGGCCCG175800.056.646248
CCAGTAG149500.054.2410285
CGCAAAA56150.052.836311
TAGCATA156100.052.1354569
GTAGCAT159050.051.347058
CAGTAGC162700.049.87066
AGTAGCA171300.047.646847
TGCCAGT175150.046.5401843
CTCACCC222000.044.830762
CCCGGCC223900.044.812096
GCCAGTA187050.043.6301884
CCGGCCC238050.042.6490757
GGCCCGG242900.041.1542639
CGGACAC160150.037.53290612-13
Produced by FastQC (version 0.11.5)