FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11658451
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT925970.794247880786221No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG772690.6627724386370024No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC392650.33679431341264804No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG309640.26559274469652955No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC302590.2595456291749221No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT270930.2323893628750509No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC210850.1808559301746004No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC202100.17335064495274716No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA195170.16740645905703938No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT191730.16445580978124796No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT182930.15690763721526987No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA182620.15664173568169562No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC171690.14726656225599782No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG166090.14246317971401176No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA166080.14245460224518677No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC161920.13888637521399713No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT158300.13578133149935614No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT153390.13156979430629334No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC152920.13116665327151952No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA152400.13072062489262082No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC139480.11963853517075296No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCA138640.11891802778945504No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG131680.11294810948727237No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA124240.10656647268149089No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC120630.10347000643567486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT30400.066.730221
ACCCGGC157200.061.3895385
ACCGTTG32900.061.373513
GACCGTT33650.060.00562
CACCCGG161400.059.7920384
TCACCCG162650.059.740253
CGCAAAA53600.054.206091
CGGCCCG177850.054.0514568
CCAGTAG144400.053.4059335
TAGCATA151350.051.239129
GTAGCAT153350.050.4772578
CAGTAGC159900.048.378046
AGTAGCA169600.045.667237
TGCCAGT171600.044.9128383
CCCGGCC220550.044.2942666
CTCACCC222150.043.632922
GCCAGTA184450.042.0924644
CCGGCCC235650.041.5164957
GGCCCGG248900.038.870399
CGGACAC159900.036.73495512-13
Produced by FastQC (version 0.11.5)