FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11807334
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT919600.7788379663012837No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG764120.6471570974446899No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC383470.32477272176767424No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG307880.2607531895006951No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC301970.2557478258851659No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT272490.23078029299416786No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC210550.17832137212346158No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC199270.1687679877608273No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA197080.1669132083499967No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT193930.1642453749508568No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT181400.1536333265409448No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA176550.1495257100375072No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC165270.13997232567487292No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC165150.13987069392633428No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA164490.1393117193093716No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG162610.1377194885822659No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT158660.13437411019286827No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT154970.13124893392530437No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA153350.12987690532003246No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC147390.1248291951426122No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCA140170.11871435160553602No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC138890.11763027962112362No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG132550.11226073557333094No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA125180.10601885235058144No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC122210.10350346657424953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT30400.064.3977361
ACCCGGC158050.060.7629785
TCACCCG164550.058.8236583
GACCGTT33350.058.701392
CACCCGG164400.058.646664
ACCGTTG33800.058.06013
CGGCCCG175600.054.9344948
CCAGTAG148950.053.4960065
CGCAAAA55450.053.1719971
TAGCATA153950.051.6685949
GTAGCAT156700.050.9120148
CAGTAGC164400.048.4112976
AGTAGCA170350.046.8042567
TGCCAGT178100.044.8998953
CCCGGCC223750.043.7052886
CTCACCC223450.043.636322
GCCAGTA188700.042.3525734
CCGGCCC237000.041.3422057
GGCCCGG242700.040.001349
CGGACAC160000.036.34315512-13
Produced by FastQC (version 0.11.5)