FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10402842
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG349420.33588898110727816No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC316990.3047148077419613No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT297240.2857296111966326No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG284500.27348295783017756No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC280070.26922450614937726No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC273950.26334149840976145No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC264490.2542478295834927No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT225350.21662349577163623No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA201540.19373551958205268No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC180580.1735871793496431No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT167990.1614847173493551No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT166420.15997551438347327No Hit
CAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCCAAGGCAA156520.15045888421644776No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT153500.14755583137761777No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC151660.14578708395263526No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG149460.14367227724885182No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA136780.13148330042886358No Hit
CAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCCAAGGCAA132910.1277631631817536No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG123660.11887136226811865No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA121610.11690074693050227No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC121220.11652584937846791No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC121200.11650662386297897No Hit
CAAGAAAGTACCCCAAGTGTCAACTCCAACTCTTGTAGAGGTCTCAAGAA120400.11573760324342136No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACA117900.11333441380730382No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT106690.10255851237575271No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT104090.10005919536219045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT27050.065.87561
ACCGTTG30900.057.66783
GACCGTT31950.055.6242832
ACCCGGC59500.051.057275
CGCAAAA46050.049.707451
CACCCGG62050.049.0339934
CGGCCCG67450.045.0430838
TCACCCG69300.044.724813
CCAGTAG82450.044.3754925
TAGCATA84600.043.7563489
GTAGCAT86500.042.903998
AGTAGCA96000.038.6573527
CAGTAGC100050.036.8065076
CCGTTGA51500.034.9687734
TAACCCC67750.034.28059
CGTTGAC54700.032.9240155
CGGACAC57300.031.0233912-13
TGCCAGT120500.030.8342273
GTAGGTC34650.029.8165422
CCCGGCC103850.029.7094926
Produced by FastQC (version 0.11.5)