FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004829288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12854941
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT745450.5798937544715297No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG501430.39006791240815497No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG354830.2760261599022508No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT313270.2436961787689263No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC309090.24044451079160922No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC278880.21694381950099964No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC248560.19335755800046067No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA247210.19230737815132717No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC247190.19229181993134004No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC209020.1625989570858396No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC206920.1609653439871875No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT202500.1575269773700245No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT199800.1554266176717575No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG198560.1544620080325534No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT171190.13317058398012097No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA167850.13057236124226476No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC164100.12765519499467168No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC159370.12397567596770767No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG152960.11898926646182195No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT136360.10607594387247674No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA133940.10419339925403003No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA128920.10028828603725214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)