FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12871346
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA404090.31394541021583916No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA325370.2527863053327912No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC226230.17576250378165578No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT217790.16920530300405257No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCA186700.14505087502115163No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA181250.14081666361855238No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG178260.13849367424354841No Hit
CTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCA177290.1377400623058381No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC175830.13660575980165554No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG164740.1279897222870087No Hit
CTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCAT153700.11941253074853245No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC146700.11397409408464353No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG146440.11377209500855621No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA146240.11361671110387368No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG139660.10850458063981809No Hit
GTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGA135050.10492298163688554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA119550.036.462231
GCGTAAC126950.035.955532
CGTAACT133750.034.4826353
GTATCGA45450.034.277964
CCCGTAT48500.032.450311
GCACCCG55050.031.8378758
TATCGAA49650.031.5696455
CCCGTCG56100.031.1146471
GTAACTA153200.030.29084
ACTAGTT153850.030.19377
ACGACTT34150.030.1654431
TGTCGAG15800.029.1601435
TAACTAG162550.028.1978075
CACCCGG66400.026.467249
CTAGTTA176350.026.2067038
CGTATCG81500.025.5265123
CCGTATC83500.024.91512
GTTACGA13050.024.3859274
ACCCGGT40550.024.01253510-11
GCGCGTT37450.023.9075290-91
Produced by FastQC (version 0.11.5)