FastQCFastQC Report
Sun 5 May 2024
EGAF00004829314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119082753
Total Bases94.6 Gbp
Sequences flagged as poor quality0
Sequence length35-131
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69523570.6212549502136774No Hit
GTTGTCAAGATGCTACCGTTCAGAGCGGCTCGTGCTGCGTCGTCTCAAGT39084340.3492533496314191No Hit
GTTGTCAAGATGCTACCGTTCAGAGCGCGTCCAATTTCCGAAGCCCCGCC35822830.32010885614997947No Hit
GTTGTCAAGATGCTACCGTTCAGAGGCAGCCGGCGTCGTACGAGGCACAG31073490.2776692779573201No Hit
GTTGTCAAGATGCTACCGTTCAGAGGCCCATTCATTGCACCCGCCAGTGA24071670.2151017870257536No Hit
GTTGTCAAGATGCTACCGTTCAGAGCCCCACCAGGTTGCTTTGTCGGACG21772620.19455772990543085No Hit
GTTGTCAAGATGCTACCGTTCAGAGTTACCCGCAGGAAGACGTATACCCC20859030.18639399047194502No Hit
GTTGTCAAGATGCTACCGTTCAGAGGTGATCCGCGCAGGCACACATACCG18819630.16817013710155893No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15565650.13909293086925092No Hit
GTTGTCAAGATGCTACCGTTCAGAGTGGATGGGATAAGTGCGTGATGGAC12455920.11130472672024104No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)