FastQCFastQC Report
Mon 6 May 2024
EGAF00004829316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977894943
Total Bases83.2 Gbp
Sequences flagged as poor quality0
Sequence length35-131
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57866440.5917449559814321No Hit
GTTGTCAAGATGCTACCGTTCAGAGCGGCTCGTGCTGCGTCGTCTCAAGT35663260.36469418576387913No Hit
GTTGTCAAGATGCTACCGTTCAGAGCGCGTCCAATTTCCGAAGCCCCGCC32523090.3325826586261424No Hit
GTTGTCAAGATGCTACCGTTCAGAGGCAGCCGGCGTCGTACGAGGCACAG30406010.3109332982817153No Hit
GTTGTCAAGATGCTACCGTTCAGAGGCCCATTCATTGCACCCGCCAGTGA22199870.22701692200079204No Hit
GTTGTCAAGATGCTACCGTTCAGAGCCCCACCAGGTTGCTTTGTCGGACG21693010.22183374763601776No Hit
GTTGTCAAGATGCTACCGTTCAGAGTTACCCGCAGGAAGACGTATACCCC20333190.207928163915252No Hit
GTTGTCAAGATGCTACCGTTCAGAGGTGATCCGCGCAGGCACACATACCG18852760.19278921662242404No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13036960.1333165703874593No Hit
GTTGTCAAGATGCTACCGTTCAGAGTGGATGGGATAAGTGCGTGATGGAC12155580.12430353676550303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)