FastQCFastQC Report
Fri 3 May 2024
EGAF00004829322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659109098
Total Bases86 Gbp
Sequences flagged as poor quality0
Sequence length35-131
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34694440.5263838734023969No Hit
GTTGTCAAGATGCTACCGTTCAGAGCGGCTCGTGCTGCGTCGTCTCAAGT22111110.3354696524003982No Hit
GTTGTCAAGATGCTACCGTTCAGAGATTGGGAGGCTTTCGTACCGCTGCC20874320.316705080590467No Hit
GTTGTCAAGATGCTACCGTTCAGAGTGGATGGGATAAGTGCGTGATGGAC20356480.3088484146519853No Hit
GTTGTCAAGATGCTACCGTTCAGAGGCCCATTCATTGCACCCGCCAGTGA14377180.21813050439792292No Hit
GTTGTCAAGATGCTACCGTTCAGAGGTGATCCGCGCAGGCACACATACCG11650400.1767598116207463No Hit
GTTGTCAAGATGCTACCGTTCAGAGTTACCCGCAGGAAGACGTATACCCC10565490.16029956242539986No Hit
GTTGTCAAGATGCTACCGTTCAGAGCGCGTCCAATTTCCGAAGCCCCGCC10133320.1537426813064565No Hit
GTTGTCAAGATGCTACCGTTCAGAGATTCAAGGGCAGCCGCGTCACGATT8903480.13508355486241522No Hit
GTTGTCAAGATGCTACCGTTCAGAGGCAGCCGGCGTCGTACGAGGCACAG8565560.1299566342809002No Hit
GTTGTCAAGATGCTACCGTTCAGAGCCCCACCAGGTTGCTTTGTCGGACG7766910.11783952040061205No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7654200.11612948483378392No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)