FastQCFastQC Report
Fri 10 May 2024
EGAF00004829324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446616359
Total Bases39.7 Gbp
Sequences flagged as poor quality0
Sequence length35-131
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTCAAGATGCTACCGTTCAGAGGCAGCCGGCGTCGTACGAGGCACAG28148130.6302529997563301No Hit
GTTGTCAAGATGCTACCGTTCAGAGATTGGGAGGCTTTCGTACCGCTGCC25385560.5683974509317067No Hit
GTTGTCAAGATGCTACCGTTCAGAGATTCAAGGGCAGCCGCGTCACGATT14748800.3302342089085904No Hit
GTTGTCAAGATGCTACCGTTCAGAGTGGATGGGATAAGTGCGTGATGGAC12017440.2690774701336007No Hit
GTTGTCAAGATGCTACCGTTCAGAGCGGCTCGTGCTGCGTCGTCTCAAGT9486530.21240892342682863No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8419930.18852712916411554No Hit
GTTGTCAAGATGCTACCGTTCAGAGCTCCCTGGTGTTCAATACCCGATGT7538690.1687956530942925No Hit
GTTGTCAAGATGCTACCGTTCAGAGTGTCTACGTCGGACCGCAAGAAGTG5038700.1128194231685096No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)