FastQCFastQC Report
Thu 16 May 2024
EGAF00004829326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285965771
Total Bases24.4 Gbp
Sequences flagged as poor quality0
Sequence length35-131
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTCAAGATGCTACCGTTCAGAGATTGGGAGGCTTTCGTACCGCTGCC17087420.5975337516880649No Hit
GTTGTCAAGATGCTACCGTTCAGAGATTCAAGGGCAGCCGCGTCACGATT16042480.5609930147898715No Hit
GTTGTCAAGATGCTACCGTTCAGAGCGGCTCGTGCTGCGTCGTCTCAAGT13363410.46730802617632167No Hit
GTTGTCAAGATGCTACCGTTCAGAGTGGATGGGATAAGTGCGTGATGGAC11071490.387161371141863No Hit
GTTGTCAAGATGCTACCGTTCAGAGCTCCCTGGTGTTCAATACCCGATGT8830400.3087922015673687No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6303040.22041239334199894No Hit
GTTGTCAAGATGCTACCGTTCAGAGTGTCTACGTCGGACCGCAAGAAGTG5846540.2044489443458602No Hit
GTTGTCAAGATGCTACCGTTCAGAGCCCCACCAGGTTGCTTTGTCGGACG3972680.13892152148517103No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3381100.11823443023186156No Hit
GTTGTCAAGATGCTACCGTTCAGAGGTGATCCGCGCAGGCACACATACCG3000570.1049275928901295No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)