FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14999768
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC207450.076.99033120
GAGCACA325750.049.207783120
AGAGCAC435500.036.91366118-119
AAGAGCA653500.025.733467120
GAAGAGC713900.022.806273118-119
GGAAGAG883950.018.757435118-119
CGGAAGA1005750.016.270658116-117
TCGGAAG1036300.015.937118116-117
GGCCTAG2950.004311669614.692311120
ACACGTC56750.014.511085120
ATCGGAA1220900.013.568768116-117
CACGTCT56300.013.4723015120
GATCGGA1244450.013.421677114-115
CACACGT51200.012.956438118-119
TAGATCG320000.012.122306120
AGATCGG1388100.011.998947114-115
CTAGATC69500.011.048201110-111
TTAGATC107150.010.314785120
ACGTCTG74050.010.047849120
GAGATCG593500.09.706488112-113
Produced by FastQC (version 0.11.5)