FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15852464
Sequences flagged as poor quality0
Sequence length53-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC88500.017.4901353
CCGATCT88600.016.1838154
AAAAAAA2401400.015.552141120
CGATCTA21300.013.5200525
CTTCCGA116750.013.1040091
GTATCAT1091300.09.829664120
CCGTATC1246700.09.720861120
TCATTAA840250.09.703453120
CATTAAA820500.09.5787325120
CGTATCA1119000.09.559532120
ATCATTA936300.09.502579120
GCCGTAT1340850.09.047244120
TATCATT1083700.08.918706120
GCGTCGG97450.08.803787120
CGCCGTA1441100.08.584399120
GTCGCCG1676250.08.450308120
TTCCGAT183400.08.439872
TCGCCGT1583200.08.185276120
TCTCGGG37050.08.096498120
ATTAAAA903450.08.04848120
Produced by FastQC (version 0.11.5)