FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15852464
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC227450.092.33329120
GAGCACA356600.057.728817120
AGAGCAC472400.044.157585118-119
AAGAGCA705500.029.183983118-119
GAAGAGC760100.027.18849116-117
GGAAGAG927250.022.022758116-117
CGATCTA21000.021.7195225
TCCGATC91250.019.9938013
CGGAAGA1081150.019.361814114-115
CCGATCT92700.019.1155724
TCGGAAG1099750.018.511175114-115
CACGTCT63950.016.559116120
ATCGGAA1307650.016.202284112-113
GATCGGA1322200.015.598431112-113
CTTCCGA121800.015.1608151
AGATCGG1431550.014.737699110-111
ACACGTC65350.014.629958118-119
CACACGT59950.014.32475118-119
GATCTAG10300.013.2338586
TAGATCG306100.013.171232110-111
Produced by FastQC (version 0.11.5)