FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11300092
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC127600.072.73024120
GAGCACA196550.049.04804120
AGAGCAC270500.035.540573120
AAGAGCA414500.023.38606118-119
GAAGAGC462650.020.50613116-117
GGAAGAG567950.017.114643116-117
TCGGAAG672350.014.36814114-115
CGGAAGA674300.014.2327585114-115
GATCGGA795850.012.272493120
ATCGGAA792500.012.206596120
AGATCGG867000.011.2151118-119
TAGATCG209200.010.930974120
CTAGATC56650.09.115879108-109
AAGATCG408150.09.036383110-111
TTAGATC98600.08.814698116-117
GAGATCG261100.08.779066110-111
CACGTCT45900.08.279204120
CACACGT43750.08.07652120
CAGATCG255750.07.8423753110-111
ACACGTC45500.07.464972118-119
Produced by FastQC (version 0.11.5)