FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13616185
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC151800.070.50285120
GAGCACA231900.044.836132120
AGAGCAC310750.034.973465120
AAGAGCA486700.022.139362118-119
GAAGAGC540600.020.937159120
GGAAGAG665750.016.941597120
CGGAAGA797600.014.257327120
TCGGAAG791500.014.191383118-119
ATCGGAA931950.012.642853120
GATCGGA944950.012.609179120
AGATCGG1049500.011.375168118-119
TAGATCG250750.010.756213114-115
CACGTCT52850.010.156544120
ACACGTC56700.010.051279120
CTAGATC62600.09.633213112-113
CACACGT52450.09.223236118-119
GAGATCG415800.09.164088120
TTAGATC102300.08.58314114-115
AAGATCG363800.08.47025120
GCTAGAT27550.08.418841120
Produced by FastQC (version 0.11.5)