FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15247842
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC174550.070.25742120
GAGCACA273650.046.334255120
AGAGCAC366250.034.949146120
AAGAGCA578150.022.801208120
GAAGAGC634100.021.053833120
GGAAGAG770150.017.300077118-119
CGGAAGA908850.014.762967120
TCGGAAG916450.014.575822116-117
GATCGGA1092550.012.495652120
ATCGGAA1078400.012.38377116-117
AGATCGG1196500.011.214022118-119
TAGATCG308700.010.62811114-115
CACACGT52300.010.133541120
CTAGATC75000.09.885449110-111
ACACGTC56350.09.762382120
CACGTCT54900.09.409232120
TTAGATC131800.09.1111555120
AAGATCG510300.09.071088120
GAGATCG392150.08.793196120
CAGATCG353150.07.826613120
Produced by FastQC (version 0.11.5)