FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16529441
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC101050.037.92076120
GAGCACA145150.029.300125120
AGAGCAC175700.026.185043120
GAAGAGC351350.014.796308120
TCGGAAG464950.014.055198120
AAGAGCA368700.013.851794120
GGAAGAG466800.012.4309225120
GATCGGA574100.012.403295120
AGATCGG599700.012.290982120
TAACCCC30250.012.0433079
TAGATCG167350.011.649274118-119
CGGAAGA526050.011.448375120
ATCGGAA608550.011.019329120
AGTGAGT38950.010.9016031
CTAGATC46250.010.422433120
GTGAGTA47750.09.6304262
TTAGATC81150.08.49657120
AAGATCG302200.08.399484120
CTACCCC25650.08.26551238-39
GAGATCG252100.07.6967607120
Produced by FastQC (version 0.11.5)