FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11835849
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC120350.064.50557120
GAGCACA172750.044.105583120
AGAGCAC233100.035.241962120
AAGAGCA381400.022.448416120
GAAGAGC413050.020.143341118-119
GGAAGAG541150.016.69245120
TCGGAAG630400.013.821349118-119
CGGAAGA660250.013.176655118-119
ATCGGAA738400.012.907088120
GATCGGA718200.012.619327118-119
TAGATCG211900.011.800245120
AGATCGG800050.011.716139120
CTAGATC50450.010.769004120
TTAGATC77950.09.992853120
GAGATCG287450.09.3029118-119
AAGATCG248650.09.030351116-117
CAGATCG268200.09.005881120
GCTAGAT21850.08.687708120
CGCTAGA6550.00341885427.9947944120
TGAGATC113400.07.965713120
Produced by FastQC (version 0.11.5)