FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12343155
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC124050.070.3781120
GAGCACA200300.046.067974120
AGAGCAC270550.034.32368120
AAGAGCA422800.022.256262118-119
GAAGAGC472200.019.762917120
GGAAGAG581100.017.195923120
TCGGAAG692450.014.676268120
CGGAAGA707950.014.07782120
ATCGGAA815050.012.427167118-119
GATCGGA816950.012.274282118-119
TAGATCG209200.011.691304120
AGATCGG899450.011.262309120
CTAGATC53800.010.210571120
TTAGATC90050.09.440884120
AAGATCG395350.08.660947114-115
GAGATCG318300.08.51999116-117
CCTAGAT18050.08.326746118-119
CAGATCG295200.08.285301120
TAAGATC157600.07.2347827108-109
ACACGTC45100.06.960136120
Produced by FastQC (version 0.11.5)