FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15158875
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC150350.067.92861120
GAGCACA224800.043.878075120
AGAGCAC304500.033.28555120
AAGAGCA476750.022.371956120
GAAGAGC533750.020.382719120
GGAAGAG682650.015.974612118-119
TCGGAAG780600.015.312548120
CGGAAGA811200.014.591407120
ATCGGAA929750.013.030035120
GATCGGA939700.012.750871118-119
AGATCGG1033900.011.498411118-119
TAGATCG264500.010.955354120
CTAGATC69350.09.699758120
AAGATCG425600.09.544036120
TTAGATC110850.08.897983114-115
GAGATCG351950.08.766201120
CCTAGAT22750.07.8180776112-113
CAGATCG365900.07.742496116-117
ATAGATC126800.07.4984064118-119
GCTAGAT28750.07.199232120
Produced by FastQC (version 0.11.5)