FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10917270
Sequences flagged as poor quality0
Sequence length51-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTGT118750.09.598881120
TAGGGAA202300.09.193194120
GAGTGTA103250.09.122422120
TAGATCT72900.09.052443120
AGTGTAG95000.08.87265118-119
AAGAGTG137200.08.657883120
GTAGGGA208700.08.623816118-119
TGTAGGG242700.08.503335120
GTCGTGT313400.08.422758120
GTGTAGG264400.08.304629120
TCGTGTA292700.08.239559120
CGTGTAG274900.07.921958120
GTGTAGA112400.07.899435120
AGATCTC88600.07.3806295120
GTAGATC121300.07.3198395120
CGTCGTG379800.07.1555643120
AGGGAAA259550.07.142299120
GAGCGTC452800.07.1016455120
AAGAGCG514300.07.0223193120
GATCGGA687150.06.975829120
Produced by FastQC (version 0.11.5)