FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14291022
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC204100.090.419075120
GAGCACA330300.052.634068120
AGAGCAC444800.040.432228120
AAGAGCA680350.026.723698120
GAAGAGC762150.024.183922120
GGAAGAG912300.020.160175118-119
CGGAAGA1047950.017.276632116-117
TCGGAAG1079500.016.93616116-117
CCGATCT78300.016.5629964
TCCGATC81700.016.5494253
CGATCTA19800.016.1657415
ATCGGAA1264250.014.458221114-115
GATCGGA1307350.014.07403114-115
AGATCGG1422900.012.840078112-113
CTTCCGA109200.012.7617251
ACACGTC52850.012.4863405120
TAGATCG353150.012.281134112-113
CTAGATC71250.011.665236112-113
CACACGT51250.011.396139120
CACGTCT51050.010.84646120
Produced by FastQC (version 0.11.5)