FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15712663
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC196100.093.37311120
GAGCACA317900.056.29021120
AGAGCAC433400.041.864555118-119
AAGAGCA653550.027.857876120
GAAGAGC767450.023.858839120
GGAAGAG930650.019.839243118-119
CGGAAGA1112900.016.477512116-117
TCGGAAG1136650.016.221413116-117
GATCGGA1320950.013.972691114-115
ATCGGAA1314150.013.968706114-115
CTAGATC76500.012.864675112-113
AGATCGG1441300.012.729893120
TAGATCG353200.011.96273116-117
TTAGATC128200.011.236216120
GAGATCG499050.010.693677112-113
AAGATCG488050.010.543267120
TGAGATC182950.09.581165108-109
CAGATCG457950.09.545113110-111
TCTAGAT36150.09.451532110-111
CCTAGAT25950.09.013557118-119
Produced by FastQC (version 0.11.5)