FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17207270
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC222400.078.88141120
GAGCACA353950.048.87328120
AGAGCAC482300.037.40259120
AAGAGCA715550.025.552092120
GAAGAGC815850.022.347414118-119
GGAAGAG1000200.018.183174118-119
TCGGAAG1207150.015.81762120
CGGAAGA1201500.015.403554120
ATCGGAA1411100.013.338102118-119
GATCGGA1451400.013.045025120
AGATCGG1592700.011.810826116-117
TAGATCG339600.010.852411118-119
CTAGATC64600.09.715464114-115
GAGATCG696150.09.300964118-119
ACACGTC58800.09.267873120
TTAGATC106900.08.708683118-119
AAGATCG462250.08.682028116-117
CACACGT50900.08.562709118-119
CAGATCG629900.08.5025835112-113
CACGTCT57700.08.233715120
Produced by FastQC (version 0.11.5)