FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16906377
Sequences flagged as poor quality0
Sequence length59-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCCC19300.011.19280546-47
GGCCCCT19000.010.42020786-87
GCGTCGG85000.09.740112120
TGGTCGC466950.08.749446120
CGGTGGT621050.08.549103120
GGTGGTC555500.08.521121120
GGCCCCA28450.08.43667846-47
AAAAAAA999700.08.359595120
GTGGTCG503300.08.355932120
GGTCGCC438100.08.243793120
TCGGTGG656400.08.216653120
CTCGGTG695350.08.101509120
AGATCTC920300.08.037806120
GTCGCCG451200.08.031037120
TAGATCT958600.08.029584120
TGGCCCC26250.07.999575684-85
GATCTCG864650.07.916789120
TCGCCGT442850.07.8573327120
ATCTCGG803850.07.84389120
TCTCGGT742650.07.827867120
Produced by FastQC (version 0.11.5)