FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13605706
Sequences flagged as poor quality0
Sequence length60-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT223250.09.647631120
AGTGTAG322750.09.591823120
AGATCTC227500.09.177314120
GTGTAGA327200.08.78294120
GTAGATC328850.08.465212120
GAGTGTA351000.08.4524118-119
GTAGGGA645100.08.277147120
AGAGTGT387850.08.275756120
AAGAGTG453050.08.183895120
CGTGTAG804150.08.176947120
GTGTAGG759600.07.9693685120
TGTAGGG696350.07.9264364120
ATCTCGG217350.07.9220943120
TAGGGAA624600.07.8956437120
GATCTCG230400.07.8379364120
GCGTCGG72000.07.6660743120
GGGAAAG603100.07.500664120
CTCGGTG198900.07.4805684120
TCGGTGG185850.07.3279243120
TCGTGTA858450.07.3278127118-119
Produced by FastQC (version 0.11.5)