FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13605706
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC123050.064.80809120
GAGCACA207900.039.70286120
AGAGCAC268400.031.179586120
AAGAGCA443400.019.18868118-119
GAAGAGC492600.018.046392120
GGAAGAG581250.016.376307120
CGGAAGA653750.014.822629120
TCGGAAG661050.014.707005120
GATCGGA777900.013.633259120
ATCGGAA768050.012.699281118-119
AGATCGG874700.012.073663120
TAGATCG243400.011.121139118-119
CTAGATC63100.010.170733118-119
AAGATCG327350.09.200959120
GAGATCG334650.09.095191120
TTAGATC93650.09.024247120
CAGATCG277400.08.509668118-119
CCTAGAT25400.07.5049496116-117
ATAGATC123500.07.3576007120
TAAGATC124300.07.080092118-119
Produced by FastQC (version 0.11.5)