FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13225372
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC176550.078.388336120
GAGCACA296100.048.229794120
AGAGCAC387950.036.29757118-119
AAGAGCA595950.024.321526120
GAAGAGC663750.021.687002120
GGAAGAG797550.018.240793118-119
TCGGAAG936800.015.920623120
CGGAAGA913900.015.906694120
ATCGGAA1109650.013.767863120
GATCGGA1124000.013.579425120
CACACGT51200.013.070156120
AGATCGG1237800.012.411607118-119
ACACGTC55650.011.769161120
TAGATCG280450.011.2453575116-117
CTAGATC75500.010.562093110-111
AAGATCG470850.010.523292116-117
GAGATCG401500.09.805336120
CAGATCG386250.09.54747118-119
CACGTCT53050.09.527962118-119
TTAGATC109000.09.1764965118-119
Produced by FastQC (version 0.11.5)