FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15622240
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC235900.097.1521120
GAGCACA394600.057.87631120
AGAGCAC540350.043.86794120
AAGAGCA797600.029.493532118-119
GAAGAGC907000.025.504198118-119
GGAAGAG1096850.021.360249116-117
CGGAAGA1253650.018.484549116-117
TCGGAAG1308650.017.870335114-115
ACACGTC68000.016.037916120
ATCGGAA1536150.015.208168114-115
GATCGGA1587200.014.881864112-113
CACACGT65550.014.680016120
CACGTCT67350.013.692356120
AGATCGG1725450.013.652396112-113
TAGATCG457350.013.070132110-111
CTAGATC106500.012.464913108-109
CAGATCG684250.011.289631110-111
GCACACG87950.011.214688120
ACGTCTG88600.011.041906120
GAGATCG564500.010.985891110-111
Produced by FastQC (version 0.11.5)