FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11802722
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC130300.073.81399120
GAGCACA207950.047.443035120
AGAGCAC282050.035.90495120
AAGAGCA427200.023.801208118-119
GAAGAGC487400.021.437181120
GGAAGAG612400.016.84942118-119
CGGAAGA715400.015.382157120
TCGGAAG717250.014.759867118-119
ATCGGAA819500.013.004083118-119
GATCGGA823150.012.824896116-117
AGATCGG904550.011.72633116-117
CTAGATC57050.011.388711118-119
TAGATCG235550.011.062649114-115
GAGATCG290750.09.790341120
AAGATCG353750.09.68518118-119
TTAGATC97400.09.318734116-117
CAGATCG266100.08.596832114-115
TAAGATC146100.08.229486118-119
ATAGATC105150.07.423295112-113
TGAGATC134650.07.3380094114-115
Produced by FastQC (version 0.11.5)